PITA Fiscal Year 2007 Projects

Biomedical and Health Engineering

Real-Time Computer Vision Tracking of Stemness
This proposal addresses the need for new stem cell engineering toolsets that can regulate adult stem cell expansion (self-renewal, without differentiation) ex vivo in order to create sufficient numbers of cells for clinical applications. The goal of the proposed work is to develop a computer vision system that tracks in real-time the spatiotemporal histories of cell divisions in vitro, and apply this system to develop novel strategies that maximize the yield of adult stem cell expansion cultures. The processing will be completed ‘on-line’ in the 15 minute intervals between successive image acquisitions, and the analysis results will be available during cell culture as feedback for process monitoring and control.